Articles

Troubleshooting Histology: Double Barrel Style – H&E and Special Stains

Why even bother to learn about troubleshooting? Wouldn’t it be more interesting to listen to one of the presentations on molecular technology? Possibly. But what would you do if your pathologist brought you a slide from the first batch of today’s H&E’s that looked sub-optimal to review alongside an optimal slide stained the previous day? …

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Vaccination Against the Covid-19 Disease – Part 3

Author’s note:  This blog is part of a series on the current status of the Covid-19 vaccination program in the United States.  All information contained herein was obtained from peer reviewed scientific journals and publications as well as legitimate vetted science-based websites.  These sources are referenced such that you may obtain further information from them.  …

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Vaccination Against the Covid-19 Disease – Part 2

Author’s note:  This blog is part of a series on the current status of the Covid-19 vaccination program in the United States.  All information contained herein was obtained from peer reviewed scientific journals and publications as well as legitimate vetted science-based websites.  These sources are referenced such that you may obtain further information from them.  …

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Validating Your New Stainer According to CAP and CLIA Requirements

Congratulations on purchasing a new autostainer! There are three parts to the start-up process:  Instrument Verification, Stain Protocol Optimization, and Validation of the Staining Protocols.  Instrument Verification – see CAP All Common Checklist (06/04/2020) COM.40350 Optimally, the company you purchase the instrument from should provide an operator manual; provide verbal procedural instructions; and test the …

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Part 2: Coronavirus Histopathology Laboratory Precautions

In the short time since part 1 of this series “Coronavirus in Histology” was published, the COVID-19 virus has continued its spread around the globe. Currently, the entire country of Italy is on lock down. The BBC reports (1) that the Italian government has implemented emergency measures related to the coronavirus outbreak, which include a …

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Part 1: Coronavirus in the Histology Laboratory

The current world health care concern over coronavirus (i.e. COVID-19) has generated many questions regarding many aspects of this fast spreading influenza disease. It is important to address the subject of the affect, if any, of this disease on how we work in and operate histopathology laboratories. An important and timely article was published online …

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#7 IHC Double Staining

The DNA of a nucleus directs the actions of the cell by instructing it to manufacture specific proteins.  This “instruction” is sent from the nucleus into the cytoplasm by “messenger RNA” (mRNA).  The mRNA exits the nucleus to bring the “message’” into the cytoplasm, binding with ribosomes to manufacture proteins.  Figure 1 illustrates this principle. …

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Rankin Gives Back to the Community

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Rankin Biomedical donated a variety of laboratory supplies to Lake Fenton High School. From syringes to block heaters and stainless steel instruments, the supplies are being put to great use in Biology, Chemistry and Physics classes!

#6 IHC Nuts and Bolts

Procedures

Immunohistochemistry Educational Series In previous installments of the IHC Educational Series, we discussed the specifics of the basic procedures found in the IHC laboratory: immunofluorescence, immunohistochemistry and in situ hybridization. Even though these are different procedures, they all require the following support procedures.Whenever a new antibody (or probe) is ordered for use by a pathologist …

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#5: In Situ Hybridization

In situ hybridization

In the previous segments of the IHC Educational Series, immunofluorescence and immunoperoxidase methods were discussed.  In both of these methods, antibodies are used to bind to and localize protein antigens in tissue sections.  Additionally, the differences between the use of polyclonal and monoclonal antibodies had been described. Another immunohistochemical method is “in situ hybridization”. The …

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