CelLock TM: An innovative standardized cell-block preparation procedure

Histology Educational Series In the previous blog we saw that every histology laboratory has a method for preparing paraffin cell-block specimens, and we reviewed those methods.  This review showed that all of these methods display various deficiencies with regard to collecting and preserving the entirety of the patient specimen with optimal retention of all of …

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CellBlockistry – The Time is Now

Histology Educational Series Every histology laboratory has a method for preparing paraffin cell-block specimens.  Certain cytology specimens, fine needle aspirates (FNAs), tiny tissue fragments, fragmented needle core biopsies and suspensions of cells for both clinical and research evaluation all require a method to get the specimen from the specimen container into a paraffin block for …

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Tissue Processing

Tissue processing begins with fixation. The fixative of choice in most histology laboratories is 10% neutral buffered formaldehyde (NBF). That is: this solution is made by diluting stock formaldehyde to a 10% concentration with phosphate buffer in the pH range of 7.0 to 7.4. Since stock formaldehyde is actually a 40% solution, the final chemical …

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Why Does the Third Container on my Tissue Processor Have White Granular Precipate in it?

In gathering more information to answer this question, my first questions would be: Are you using 10% neutral buffered formalin (NBF)? Is the first alcohol after the NBF 95% alcohol? I’m going to assume your answers would be YES to both of these questions. Let’s review tissue processing for a moment, so we’re all on …

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