Tissue Processing

Tissue Processing

Tissue processing begins with fixation. The fixative of choice in most histology laboratories is 10% neutral buffered formaldehyde (NBF). That is: this solution is made by diluting stock formaldehyde to a 10% concentration with phosphate buffer in the pH range of 7.0 to 7.4. Since stock formaldehyde is actually a 40% solution, the final chemical …

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Why Does the Third Container on my Tissue Processor Have White Granular Precipate in it?

In gathering more information to answer this question, my first questions would be: Are you using 10% neutral buffered formalin (NBF)? Is the first alcohol after the NBF 95% alcohol? I’m going to assume your answers would be YES to both of these questions. Let’s review tissue processing for a moment, so we’re all on …

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