American Optical AO 860 Sliding Microtome

Sliding

Sliding microtomes are suited for sectioning specimens embedded in all forms of media, especially tough tissue blocks. They were developed mainly for cutting celloidin-embedded tissue blocks. Sliding microtome sections are cut in a perfectly flat plane, thereby making excellent serial tissue sections. The block remains stationary while the knife is moved backward and forward during the process of sectioning.  Typically, a large knife (120 mm to 180 mm in length) usually wedge shaped is used. Sectioning with a sliding microtome is a slower process than with a rotary microtomes and is more dangerous due to a movable exposed knife. However, it is more stable with less vibration than the ordinary rotary microtome. Cut thickness is between 1 and 60 µm.